In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme L Extract on human lung cancer cell line NCI-H157

ObjectiveTo investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract (ESC) in vitro.MethodsESC was first extracted with ethanol, and then washed using a polyamide column with 60% ethanol. ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions. Subsequently, the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined.ResultsThe results showed that ESC was rich in isomers of Chamaejasminor, neochamaejasmine and Sikokianin. ESC had significant cytotoxicity against NCI-H157 cells, with an IC50 of approximately 18.50 μg · mL−1. ESC caused significant increase in total apoptotic rate, the activity of caspase 3 and 8, and Fas protein expression (P<0.05).ConclusionThe inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway.