Effects of Bushen Huoxue Yin () on brain NF-κB and NO content in the parkinson's disease model mouse

ObjectiveTo observe the effects of Bushen Huoxue Yin (, BSHXY) on nuclear transcription factor kappa B (NF-κB) and nitric oxide (NO) in the brain of the Parkinson's disease (PD) model mouse.MethodsForty-five C57BL/6 mice were randomly divided into three groups; normal, model and BSHXY treatment groups. Concentrations of NF-κB and NO in mouse brain tissue were determined by ELISA and spectrophotometry, respectively.ResultsNF-κB concentration in brain tissue in the model group was 14.04±4.38 μg · L−1, which was higher than that in normal (P<0.01) and BSHXY (P<0.05) groups. NO content in brain tissue in the model group was 5.93±0.79 μmol · gprot−1, which was also higher than that in model (P<0.01) and BSHXY (P<0.01) groups. However, there were no significant differences in the content of NF-κB and NO between BSHXY and normal groups (P>0.05).ConclusionThe mechanism of BSHXY for treatment of PD is possibly related to inhibition of NF-κB activation and decreased NO content in the brain.