Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4209371 | Journal of Cystic Fibrosis | 2008 | 4 Pages |
Abstract
Individual PCR amplification tests have been developed for three UK CF epidemic strains, the Liverpool epidemic strain (LES), Midlands 1 and the Manchester epidemic strain (MES). We report a simple diagnostic multiplex PCR test that can be used to screen for all three of these strains. To evaluate the test, we screened collections of LES, MES and Midlands 1 isolates, along with various CF and non-CF non-epidemic Pseudomonas aeruginosa strains. The test was 100% sensitive and 100% specific in the identification of these UK CF epidemic strains.
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Authors
Joanne L. Fothergill, Anna L. Upton, Tyrone L. Pitt, C. Anthony Hart, Craig Winstanley,