| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4255995 | Transplantation Proceedings | 2016 | 5 Pages |
•We investigated the effects of resveratrol on warm I/R injury in rats.•We revealed the cytoprotective effect of resveratrol on NHBD grafts.•Resveratrol could reduce inflammatory cytokines on NHBD grafts.•Resveratrol increased the value of ATP contents on NHBD grafts.•Resveratrol preserved microcirculation of liver grafts subjected to warm I/R injury.
BackgroundSuccessful liver transplantation from non–heart-beating donors (NHBDs) might enlarge donor source. Some studies have reported that resveratrol (RES), an activator of sirtuins, has cytoprotective effects on ischemia-reperfusion (I/R) injury. The purpose of this study was to investigate the effects of RES on warm I/R injury in rats.MethodsMale Wister rats were divided into 5 groups: (1) the heart-beating (HB) group, whose livers were retrieved from HB donors; (2) the NHB group, whose livers were retrieved under apnea-induced NHB conditions; (3) the ethanol group, retrieved in the same manner as the NHB group with ethanol (10 μL) as a solvent; (4) the RES-1 group, retrieved in the same manner as the NHB group and pretreated with RES (0.4 mg/kg, dissolved in 10 μL ethanol); and (5) the RES-2 group, retrieved in the same manner as the NHB group and pretreated with RES (2 mg/kg, dissolved in 10 μL ethanol). The resected livers were perfused for 60 minutes with Krebs-Henseleit bicarbonate buffer after 6 hours of cold preservation, after which the perfusate and liver tissues were investigated.ResultsThe bile production, portal vein flow volume, tumor necrosis factor-α level, and adenosine triphosphate level in the RES-2 group were significantly improved compared with in the NHB group. Histology revealed numerous well-preserved sinusoidal endothelial cells in the RES-2 group.ConclusionsRES might reduce warm I/R injury and improve the viability of liver grafts from NHBDs. We considered that this method may represent a promising approach for clinical liver transplantation from NHBDs.
