Modulation of the Reaction of Vascular Smooth Muscle Cells to Angiotensin II Induced by Catalase and Aminotriasol During Ischemia–Reperfusion

BackgroundWe investigated the influence of catalase and aminotriasol on reactions of the smooth muscle cells induced by angiotensin II (ANG II) after ischemia–reperfusion (I/R).Materials and MethodsExperiments were performed on perfused male Wistar rat tail arteries. Using classical pharmacometric methods we analyzed the influence of ANG II on vascular contraction, in the presence of catalase and aminotriazole, and after I/R.ResultsA reduction in maximal response and increased EC5 value were observed after ischemia, while an increased maximal response and decrease EC50 value were observed after reperfusion. Catalase decreased and aminotriasol increased maximal responses to ANG II. In the presence of catalase, reduction of the maximal response and increase in EC50 value were observed after reperfusion. In the presence of aminotriasol, we observed increased maximal response and decreased EC50 value after I/R.ConclusionIschemia reduced and reperfusion increased the responses of vascular smooth muscle cells to ANG II. Catalase decreased and aminotriasol increased hyperreactivity of arteries to ANG II after reperfusion. These results suggested that antioxidative system modulates reactions induced by ANG II. Reperfusion impairs the balance between antioxidants and the production of reactive oxygen species.