| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4260927 | Transplantation Proceedings | 2009 | 5 Pages |
ObjectiveWe investigated the relevance of donor bone marrow cell infusion (DBMI) and serum levels of interferon-γ (IFN-γ), interleukin-10 (IL-10), and soluble CD30 (sCD30) in kidney recipients.Patients and MethodsWe analyzed the allograft outcomes correlated with sCD30, IFN-γ, and IL-10 levels using pre- and posttransplantation sera from 40 live donor renal transplants (20 patients with DBMI [2.1 × 109 ± 1.3 × 109 mononuclear cells/body] and 20 controls).ResultsPatients with acute rejection episodes (ARE)—3/20 DBMI and 6/20 controls—showed increased sCD30 and IFN-γ as well as decreased IL-10 posttransplantation compared with nonrejectors. Significant differences were observed for sCD30 and IFN-γ levels: 59.54 vs 30.92 ng/mL (P = .02) and 11.91 vs 3.01 pg/mL (P = .01), respectively. Comparison of pre- and posttransplant levels of IFN-γ, IL-10, and sCD30 in ARE patients showed higher levels in posttransplant sera except for IFN-γ in controls (6.37 vs 11.93; P = .01). Increased IFN-γ and IL-10 were correlated with rejection (r = .93; P = .008). sCD30 correlated with serum creatinine among ARE patients in control and DBMI groups (r = .89; P = .019; and r = 1.00; P < .0001, respectively).ConclusionsHigher levels of sCD30, IFN-γ, and IL-10 posttransplantation in rejecting patients provided evidence for coexistence of cellular and humoral responses in ARE. There appeared to be a down-regulatory effect of infusion on alloresponses.
