| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4312011 | Behavioural Brain Research | 2017 | 14 Pages |
•An elevated platform with steep slopes on two opposite sides is proposed to test anxiety in mice.•The test apparatus offers a 3-dimensional open-field that compares to a real world navigation space.•BL6J and CD-1, not BALB/c, crossed onto downward slopes and reached the stands from upward slopes.•Crossings onto downward slopes decreased with further elevation of the platform in BL6J and CD-1.•Times of the day did not affect BALB/c behavior in downward shallow and downward steep slopes.
When exposed to an unfamiliar open space, animals experience fear and attempt to find an escape route. Anxiety emerges when animals are confronted with a challenging obstacle to this fear motivated escape. High anxiety animals do not take risks; they avoid the challenge. The present experiments investigated this risk avoidant behavior in mice. In experiment 1, BALB/c, C57BL/6J and CD-1 mice were exposed to a large platform with downward inclined steep slopes attached on two opposite sides. The platform was elevated 75 and 100 cm from the ground, in a standard (SPDS) and in a raised (RPDS) configuration, respectively. In experiment 2, the platform was elevated 75 cm from the ground. Mice had to climb onto a stand at the top of upward inclined slopes (SPUS). In experiment 3, BALB/c mice were exposed to SPDS with steep or shallow slopes either in early morning or in late afternoon. In all 3 test configurations, mice spent more time in the areas adjacent to the slopes than in the areas adjacent to void, however only C57BL/6J and CD-1 crossed onto the slopes in SPDS, and crossed onto the stands in SPUS whereas BALB/c remained on the platform in SPDS and explored the slopes in SPUS. Elevation of the platform from the ground reduced the crossings onto the slopes in C57BL/6J and CD-1, and no differences were observed between BALB/c and C57BL/6J. BALB/c mice demonstrated no difference in anxiety when tested early morning or late afternoon; they crossed onto shallow slopes and avoided the steep one.
