| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4318907 | Brain Research Bulletin | 2013 | 8 Pages |
AimsThis study analyzes how age and inflammation modify the response of the vesicular glutamate transporters (VGLUTs), VGLUT1–3 to global brain ischemia/reperfusion (I/R) in brain areas with different I/R vulnerabilities.ResultsGlobal ischemia was induced in 3- and 18-month-old male Sprague–Dawley rats and CA1 and CA3 hippocampal areas, dentate gyrus and cerebral cortex of sham-operated and I/R animals were removed 48 h after insult. Real-time PCR analysis revealed that I/R challenge resulted in a significant decrease of the VGLUT mRNA levels in young animals. Western blot assays showed a lessened age-dependent response to the ischemic damage in VGLUT1 and VGLUT3, while VGLUT2 presented an age and structure-dependent response to challenge. The use of the anti-inflammatory agent meloxicam following challenge showed that COX2 inhibition promotes the expression of VGLUTs in both sham and injured animals, which results in a lessened response to I/R injury.ConclusionsVGLUT1 and VGLUT3 presented an age-dependent response to ischemic damage, while this VGLUT response was age both and structure-dependent. In addition, COX-2 inhibition resulted in an increase of VGLUT1 and VGLUT2 protein amounts both in sham and injured animals together with a lessening of the transporters’ response to ischemia.
► Both age and inflammation play critical roles in the response to ischemia. ► We have analyzed the role of vesicular glutamate transporters in cerebral ischemia. ► The transcription of VGLUTs is modified in an age and inflammation-dependent way.
