Article ID Journal Published Year Pages File Type
4321110 Neuron 2014 13 Pages PDF
Abstract

•The NuRD chromatin remodeling complex drives synapse differentiation in the brain•NuRD complex represses a program of genes in neurons as they mature in the brain•NuRD complex decommissions the promoters of repressed genes in neurons•In vivo screen uncovers specific NuRD targets that control synapse differentiation

SummaryPrecise control of gene expression plays fundamental roles in brain development, but the roles of chromatin regulators in neuronal connectivity have remained poorly understood. We report that depletion of the NuRD complex by in vivo RNAi and conditional knockout of the core NuRD subunit Chd4 profoundly impairs the establishment of granule neuron parallel fiber/Purkinje cell synapses in the rodent cerebellar cortex in vivo. By interfacing genome-wide sequencing of transcripts and ChIP-seq analyses, we uncover a network of repressed genes and distinct histone modifications at target gene promoters that are developmentally regulated by the NuRD complex in the cerebellum in vivo. Finally, in a targeted in vivo RNAi screen of NuRD target genes, we identify a program of NuRD-repressed genes that operate as critical regulators of presynaptic differentiation in the cerebellar cortex. Our findings define NuRD-dependent promoter decommissioning as a developmentally regulated programming mechanism that drives synaptic connectivity in the mammalian brain.

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Life Sciences Neuroscience Cellular and Molecular Neuroscience
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