Article ID Journal Published Year Pages File Type
4321218 Neuron 2012 17 Pages PDF
Abstract

SummaryNeurites are the characteristic structural element of neurons that will initiate brain connectivity and elaborate information. Early in development, neurons are spherical cells but this symmetry is broken through the initial formation of neurites. This fundamental step is thought to rely on actin and microtubule dynamics. However, it is unclear which aspects of the complex actin behavior control neuritogenesis and which molecular mechanisms are involved. Here, we demonstrate that augmented actin retrograde flow and protrusion dynamics facilitate neurite formation. Our data indicate that a single family of actin regulatory proteins, ADF/Cofilin, provides the required control of actin retrograde flow and dynamics to form neurites. In particular, the F-actin severing activity of ADF/Cofilin organizes space for the protrusion and bundling of microtubules, the backbone of neurites. Our data reveal how ADF/Cofilin organizes the cytoskeleton to drive actin retrograde flow and thus break the spherical shape of neurons.

► Actin retrograde flow and dynamics direct neurite growth out of the neuronal sphere ► ADF/Cofilin is a key regulator of neuritogenesis during brain development ► ADF/Cofilin drives actin retrograde flow and dynamics ► ADF/Cofilin activity enables the growth of bundled microtubules to form neurites

Related Topics
Life Sciences Neuroscience Cellular and Molecular Neuroscience
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