Analysis of NPR-1 Reveals a Circuit Mechanism for Behavioral Quiescence in C. elegans

•NPR-1 and its ligands are required for locomotion quiescence during lethargus•Increased activity in the RMG circuit promotes locomotion arousal•Arousal is mediated by increased secretion of PDF-1 from the RMG circuit•PDF-1 enhances the sensitivity of mechanosory neurons, increasing motility

SummaryAnimals undergo periods of behavioral quiescence and arousal in response to environmental, circadian, or developmental cues. During larval molts, C. elegans undergoes a period of profound behavioral quiescence termed lethargus. Locomotion quiescence during lethargus was abolished in mutants lacking a neuropeptide receptor (NPR-1) and was reduced in mutants lacking NPR-1 ligands (FLP-18 and FLP-21). Wild-type strains are polymorphic for the npr-1 gene, and their lethargus behavior varies correspondingly. Locomotion quiescence and arousal were mediated by decreased and increased secretion of an arousal neuropeptide (PDF-1) from central neurons. PDF receptors (PDFR-1) expressed in peripheral mechanosensory neurons enhanced touch-evoked calcium transients. Thus, a central circuit stimulates arousal from lethargus by enhancing the sensitivity of peripheral mechanosensory neurons in the body. These results define a circuit mechanism controlling a developmentally programmed form of quiescence.