Article ID Journal Published Year Pages File Type
4321664 Neuron 2010 14 Pages PDF
Abstract

SummaryFasting has been used to control epilepsy since antiquity, but the mechanism of coupling between metabolic state and excitatory neurotransmission remains unknown. Previous work has shown that the vesicular glutamate transporters (VGLUTs) required for exocytotic release of glutamate undergo an unusual form of regulation by Cl−. Using functional reconstitution of the purified VGLUTs into proteoliposomes, we now show that Cl− acts as an allosteric activator, and the ketone bodies that increase with fasting inhibit glutamate release by competing with Cl− at the site of allosteric regulation. Consistent with these observations, acetoacetate reduced quantal size at hippocampal synapses and suppresses glutamate release and seizures evoked with 4-aminopyridine in the brain. The results indicate an unsuspected link between metabolic state and excitatory neurotransmission through anion-dependent regulation of VGLUT activity.

► Cl− acts as an allosteric activator of VGLUTs ► Ketone bodies inhibited vesicular glutamate uptake by competing with Cl− ► Acetoacetate reduced quantal size at hippocampal synapses ► Acetoacetate suppressed 4AP-evoked glutamate release and seizures

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