Article ID Journal Published Year Pages File Type
4322222 Neuron 2011 16 Pages PDF
Abstract

SummaryHow the mitosis of neuroepithelial stem cells is restricted to the apical ventricular area remains unclear. In zebrafish, the mosaic eyesrw306 (moe/epb41l5rw306) mutation disrupts the interaction between the putative adaptor protein Moe and the apicobasal polarity regulator Crumbs (Crb), and impairs the maintenance of neuroepithelial apicobasal polarity. While Crb interacts directly with Notch and inhibits its activity, Moe reverses this inhibition. In the moerw306 hindbrain, Notch activity is significantly reduced, and the number of cells that proliferate basally away from the apical area is increased. Surprisingly, activation of Notch in the moerw306 mutant rescues not only the basally localized proliferation but also the aberrant neuroepithelial apicobasal polarity. We present evidence that the Crb⋅Moe complex and Notch play key roles in a positive feedback loop to maintain the apicobasal polarity and the apical-high basal-low gradient of Notch activity in neuroepithelial cells, both of which are essential for their apically restricted mitosis.

► We have revealed how the mitosis of neuroepithelial cells is apically restricted ► Notch maintains neuroepithelial apicobasal polarity in R-Ras-dependent manner ► Crumbs inhibits the Notch activation, while Mosaic eyes counteracts this inhibition ► Notch signaling restricts neuroepithelial mitosis to the apical area

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