Casein Kinase 2 Regulates the NR2 Subunit Composition of Synaptic NMDA Receptors

SummaryN-methyl-D-aspartate (NMDA) receptors (NMDARs) play a central role in development, synaptic plasticity, and neurological disease. NMDAR subunit composition defines their biophysical properties and downstream signaling. Casein kinase 2 (CK2) phosphorylates the NR2B subunit within its PDZ-binding domain; however, the consequences for NMDAR localization and function are unclear. Here we show that CK2 phosphorylation of NR2B regulates synaptic NR2B and NR2A in response to activity. We find that CK2 phosphorylates NR2B, but not NR2A, to drive NR2B-endocytosis and remove NR2B from synapses resulting in an increase in synaptic NR2A expression. During development there is an activity-dependent switch from NR2B to NR2A at cortical synapses. We observe an increase in CK2 expression and NR2B phosphorylation over this same critical period and show that the acute activity-dependent switch in NR2 subunit composition at developing hippocampal synapses requires CK2 activity. Thus, CK2 plays a central role in determining the NR2 subunit content of synaptic NMDARs.

► CK2 phosphorylation of NR2B in the PDZ binding domain (S1480) reduces synaptic NR2B ► CK2 activity, not direct phosphorylation, increases synaptic NR2A ► CK2 activity and phosphorylation of NR2B on S1480 increase during development ► The acute activity-dependent NR2B to NR2A switch requires CK2 activity