| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4324308 | Brain Research | 2014 | 9 Pages |
•hUC-MSCs can pass through the blood–brain barrier and migrate toward the lesion site.•hUC-MSC treatment increased the number of mature oligodendrocytes.•hUC-MSC treatment decreased reactive astrocyte and activated microglia counts.•hUC-MSCs treatment improved the long-term functional outcome of rats.
Periventricular white matter damage (PWMD) also termed periventricular leucomalacia in the preterm infant is of particular importance because no targeted therapy is presently available. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have been studied in a variety of adult brain injury-related neurological disorders. Our aim was to determine whether hUC-MSC transplantation improves glial cell function in cerebral white matter and long-term behavioral function in a PWMD rat model. Rats on postnatal day (P) 3 underwent a permanent ligation of the left common carotid artery followed by 6% O2 for 4 h. Immediately after the hypoxic–ischemic (HI), rats received a single intraperitoneal injection of hUC-MSCs, which were co-cultured with 5-bromodeoxyuridine (BrdU). BrdU+ cells in the brain were tested 24 h after transplantation. Second, rats received hUC-MSC treatment once a day for 3 consecutive days. Glial cells (oligodendrocytes, astrocytes and microglia) were examined on 7 and 18 days post-HI, and behavioral outcomes were tested 27 days post-HI. Significantly, hUC-MSCs migrated mainly into the injured hemisphere. In addition, hUC-MSC treatment improved the long-term functional outcomes of rats, increased mature oligodendrocyte counts, and decreased the number of reactive astrocytes and activated microglia quantities after HI-induced damage in the premature brain. These results suggest that hUC-MSCs can pass through the blood–brain barrier and migrate towards the lesion site to improve brain damage. Therefore, hUC-MSCs have the potential to be utilized as a novel therapeutic strategy for PWMD.
