| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4324643 | Brain Research | 2013 | 10 Pages |
•Polyhydroxy pregnane glycoside (PPG), a progesterone like molecule was isolated from Wattakaka volubilis (L.f) Stapf.•PPG was assessed to neuroprotective studies using middle cerebral artery occlusion in rats.•The brain antioxidant mechanisms were studied using various enzymes.•PPG (10 mg/kg) exhibit neuroprotective effect through its anti-oxidative action in MCAO rats.•We report PPG as a potent bioactive molecule for Ischemic stroke/reperfusion.
Wattakaka volubilis (L.f) (Stapf.) is well known for its rich steroid pregnane contents and this plant is known for its potential use as alternative biological activities. However, little is known about its neuroprotective effect in ischemia–reperfusion (I/R)-induced cerebral injury. Hence, the present study was carried out to investigate the effect of W. volubilis(L.f) (Stapf.) sapogenin glycosides mixture (WVSM) and polyhydroxy pregnane glycoside (PPG) isolated from it on neuroprotection and the potential mechanisms in the rat I/R model induced by middle cerebral artery occlusion (MCAO). The rats were pretreated with WVSM (50 mg/kg) and PPG (5 mg/kg and 10 mg/kg) (p.o.) once a day for 5 days before MCAO. Neurological deficit score (NDS), rotarod performance, brain water content, 2,3,5-triphenyltetrazolium chloride (TTC) staining and indices of oxidative stress [superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) and nitric oxide synthase (NOS)] were measured at 24 h after ischemia. The results indicated that pretreatment with WVSM and PPG (10 mg/kg) significantly diminished the volume of infarction and brain water content, and improved NDS. Moreover, WVSM and PPG (10 mg/kg) markedly reversed the level of MDA, NO, NOS and SOD to their normal state in serum or cerebral ischemic section. These findings demonstrate that WVSM and PPG (10 mg/kg) exhibit neuroprotective effect through its anti-oxidative action in MCAO rats.
