Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4327596 | Brain Research | 2009 | 10 Pages |
Abstract
Recently, we cloned purpurin cDNA as an upregulated gene in the axotomized fish retina. The retina-specific protein was secreted from photoreceptors to ganglion cell layer during an early stage of optic nerve regeneration in zebrafish retina. The purpurin worked as a trigger molecule for axonal regrowth in adult injured fish retina. During zebrafish development, purpurin mRNA first appeared in ventral retina at 2Â days post-fertilization (dpf) and spread out to the outer nuclear layer at 3Â dpf. Here, we investigated the role of purpurin for zebrafish retinal development using morpholino gene knockdown technique. Injection of purpurin morpholino into the 1-2 cell stage of embryos significantly inhibited the transcriptional and translational expression of purpurin at 3Â dpf. In the purpurin morphant, the eyeball was significantly smaller and retinal lamination of nuclear and plexiform layers was not formed at 3Â dpf. Retinal cells of purpurin morphants were still proliferative and undifferentiated at 3Â dpf. The visual function of purpurin morphant estimated by optomotor response was also suppressed at 5Â dpf. By contrast, the control morphants with random sequence morpholino showed retinal lamination with distinct layers and differentiated cells at 3Â dpf. These results strongly suggest that purpurin is a key molecule for not only optic nerve regeneration in adult but also cell differentiation during early development in embryo.
Keywords
GAP-43DPFGFAPONLINLFGFHDAChpfPKCHSPGbromo-deoxyuridineBrdURetinoic acidDevelopmentgrowth associated protein-43days post-fertilizationhours post-fertilizationRetinafibroblast growth factorouter nuclear layerinner nuclear layermorpholinoNeurogenesisHeparan sulfate proteoglycanhistone deacetylaseGlial fibrillary acidic proteinProtein kinase CPurpurinZebrafish
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Authors
Mikiko Nagashima, Kazuhiro Mawatari, Masayuki Tanaka, Tomomi Higashi, Hikaru Saito, Ken-ichiro Muramoto, Toru Matsukawa, Yoshiki Koriyama, Kayo Sugitani, Satoru Kato,