Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4330013 | Brain Research | 2008 | 6 Pages |
Abstract
Hypoxic ischemic (HI) injury in neonates damages brain tissues. We examined the mechanism of hyperbaric oxygen preconditioning (HBO-PC) in neonatal HI rat model. Seven-day-old rat pups were subjected to left common carotid artery ligation and hypoxia (8% oxygen at 37 °C) for 90 min. HBO (100% O2, 2.5 atmospheres absolute for 2.5 h) were administered by placing pups in a chamber 24 h before HI insult. Brain injury was assessed by the survival rate, 2,3,5-triphenyltetrazolium chloride (TTC), Nissl, TUNEL straining and caspase-3,caspase-9 activities after HI. In HBO preconditioned animals, survival rate was increased, infarct ratio was decreased, and the positive stained TUNEL cells were reduced, accompanied by the suppression of caspase-3 and -9 activities. These results indicate that a single HBO-PC appears to provide brain protection against HI insult via inhibition of neuronal apoptosis pathways.
Keywords
CATHbOHIF-1αNBTTTCIPCBCIPATAHIBDhyperbaric oxygen preconditioningHypoxic ischemiaPBSatmosphere absolute2,3,5-triphenyltetrazolium chloride5-bromo-4-chloro-3-indolylphosphateBDNFischemic preconditioningROSnitroblue tetrazoliumPolyunsaturated fatty acidPUFAHyperbaric oxygenanalysis of varianceANOVATerminal deoxynucleotidyl transferase-mediated dUTP nick end labelingTUNELApoptosisSODSuperoxide dismutasehypoxia-inducible factor-1αbrain derived neurotrophic factorPhosphate-buffered salineCatalaseReactive oxygen species
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Authors
Zhang Li, Wenwu Liu, Zhimin Kang, Shijun Lv, Cuihong Han, Liu Yun, Xuejun Sun, John H. Zhang,