Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4330207 | Brain Research | 2008 | 5 Pages |
Abstract
There is increasing evidence that the sodium-calcium exchanger (NCX) subtypes, NCX1, NCX2 and NCX3 play an important role in intracellular calcium homeostasis/dysregulation following cerebral ischemia. In the present study we examined NCX1, NCX2 and NCX3 protein levels in the rat hippocampus at 3, 6, 12, 18, 24 and 48Â h following a 3Â min and 8Â min duration of global cerebral ischemia. We observed that NCX1 protein levels were significantly increased by 22.3% and 20.6% at the 6 and 12Â h respective time points following a 3Â min duration of global ischemia, while NCX2 and NCX3 protein levels remained relatively constant. Following a 8Â min duration of global ischemia, NCX1 protein levels remained relatively constant, while NCX2 protein levels were down-regulated by 6.9%, 10.8%, 14.4% and 10.3% at the 6, 18, 24 and 48Â h respective time points, and NCX3 protein levels were up-regulated by 22.1% at the 18Â h time point. Taken together, our results show that NCX subtype protein expression is sensitive to cerebral ischemia, and indicates that changes in NCX activity may be playing an important role in calcium maintenance and neuronal outcome following ischemia.
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Authors
Christina Bojarski, Bruno P. Meloni, Stephen R. Moore, Bernadette T. Majda, Neville W. Knuckey,