| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4335143 | Journal of Neuroscience Methods | 2012 | 10 Pages |
Abstract
⺠We report a method for spatially resolved analysis of VMAT2 mediated uptake in live cells. ⺠Uptake of the fluorescent substrate was measured in HEK293 cell lines stable expressing DAT and mCherry-VMAT2. ⺠Uptake into the mCherry positive compartment is sensitive to tetrabenazine. ⺠VMAT2 activity can be quantified by analyzing confocal images. ⺠We have developed an assay amenable to high content imaging that is low cost, uses no animals, and is flexible compared to radioactive assays.
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Authors
Alison I. Bernstein, Kristen A. Stout, Gary W. Miller,