| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4335393 | Journal of Neuroscience Methods | 2011 | 8 Pages |
This article describes a perfusion system for biophysical single cell experiments at the physiological temperature. Our system regulates temperature of test solutions using a small heat exchanger that includes several capillaries. Water circulating inside the heat exchanger warms or cools test solutions flowing inside the capillaries. Temperature-controlled solutions are delivered directly to a single cell(s) through a multibarreled manifold that switches solutions bathing a cell in less than 1 s. This solution exchange is optimal for patch clamp, single-cell microamperometry, and microfluorometry experiments. Using this system, we demonstrate that exocytosis from pancreatic β cells and activation of TRPV1 channels are temperature sensitive. We also discuss how to measure local temperature near a single cell under investigation.
► We designed a local perfusion system for single cell biophysical experiments. ► A heat exchanger in the system warms or cools solution. ► Temperature variation between solutions applied to a cell was minimal (1 °C). ► Switch between solutions is quick (<1 s). ► Our system is suitable for patch clamp, Ca2+ imaging, and microamperometry with single cells.
