| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4335952 | Journal of Neuroscience Methods | 2008 | 4 Pages |
Abstract
A Ti:sapphire femtosecond laser micro-manipulation system was used to perforate vital cells. Propidium iodide was successfully transferred into both astrocytes and PC12 cells via the channel of a femtosecond laser, which induced transiently recoverable opening of the cell membrane. The transfection efficiency reached almost 100%. This method also had the advantage of contact-free, non-disruptive and stable transfection.
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Authors
Ming Lei, Hanpeng Xu, Hao Yang, Baoli Yao,