Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4336325 | Journal of Neuroscience Methods | 2008 | 7 Pages |
Abstract
Synapses are individually operated, computational units for neural communication. To manipulate physically individual synapses in a living organism, we have developed a laser ablation technique for removing single synapses in live neurons in C. elegans that operates without apparent damage to the axon. As a complementary technique, we applied microfluidic immobilization of C. elegans to facilitate long-term fluorescence imaging and observation of neuronal development. With this technique, we directly demonstrated the existence of competition between developing synapses in the HSNL motor neuron.
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Authors
Peter B. Allen, Allyson E. Sgro, Daniel L. Chao, Byron E. Doepker, J. Scott Edgar, Kang Shen, Daniel T. Chiu,