Automatic positioning and sensing microelectrode array (APSMEA) for multi-site electrophysiological recordings

Technological improvement of measurements for the electrical recordings from individual neurons within network is essential in neuroscience today. Here, we present a novel automatic positioning and sensing microelectrode array (APSMEA), which simultaneously positioned desired number of neurons onto 48 recording microelectrodes automatically and scathelessly by use of negative dielectrophoretic (DEP) forces, and facilitated the measurement of the electrophysiological activities of neuronal populations after functional synaptic connections formed between neurons. The results of multi-site electrophysiological recordings during drug administration also demonstrated the application of APSMEA in bioassay with cultured rat cortical neurons. Therefore, this device should benefit the investigation of neuronal networks in vitro with more comprehensive electrophysiological experiments, and also promise the possibility of a modular device for both cell manipulation and cell-based biosensor on microchip.