Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4337809 | Neuroscience | 2013 | 10 Pages |
Abstract
Organic cation transporters (OCTs) are expressed mainly in the kidney and liver. OCTs transport intrinsic organic cations, including monoamine, dopamine, serotonine and choline, across the plasma membrane. Here, we demonstrate that OCT2 (SLC22A2) is expressed in cholinergic neurons, motoneurons in the anterior horn of the spinal cord, and is implicated in acetylcholine (Ach) recycling in presynaptic terminals. Application of rabbit anti-peptide antibody revealed that OCT2 was expressed in the anterior horn of the spinal cord. Double immunostaining of muscle sections with anti-OCT2 and alpha-bungarotoxin (BTX) revealed that OCT2 was localized in the neuromuscular junctions (NMJs). Immunoelectron microscopy revealed that OCT2 was localized both in synaptic vesicles (SVs) in presynaptic terminals around the motoneurons (C-terminals) and in SVs in nerve terminals in NMJs. The similarity in the distribution of OCT2 in cholinergic neurons and that of vesicular acetyl choline transporter (VAchT), and the fact that OCT2 can transport choline suggest that OCT2 could work as a low-affinity and high-capacity choline transporter at presynaptic terminals in cholinergic neurons in a firing-dependent manner.
Keywords
AChRm-maleimidobenzoyl-N-hydroxysuccinimide esterNMJsOCTsHemicholiniumOCT2SVSDPXHC-3VAChTMBSalpha-bungarotoxinFCSPBSNGSHRPBTXDMEMDABKLH3,3′-diaminobenzidineAChneuromuscular junctionsAcetylcholinephosphate bufferorganic cation transporterCNSnormal goat serumfetal calf serumcentral nervous systemendoplasmic reticulumtransmembraneCortexPhosphate-buffered salineDulbecco’s modified eagle’s mediumCholinergic neuronsynaptic vesicleHorseradish peroxidaseChATcentral canalOrganic cation transporterskeyhole limpet hemocyanincholine acetyltransferaseSynaptic vesicles
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
T. Nakata, T. Matsui, K. Kobayashi, Y. Kobayashi, N. Anzai,