Ultrastructure, synaptic organization, and molecular components of bushy cell networks in the anteroventral cochlear nucleus of the rhesus monkey

Bushy cells (BCs) process auditory information in the ventral cochlear nucleus (VCN). Yet, most neuroanatomical findings come from studies in cats and rodents, and the ultrastructural morphological features of BCs in humans and higher nonhuman primates are unknown. In this study, we combined histological, immunocytochemical, and ultrastructural methods to examine the morphology and synaptic organization of BCs in the rhesus monkey VCN. We observed that BCs were organized in a complex neural network that appears to interconnect the cells. The fine structure of BC somata and dendrites, as well as their synaptic inputs, are similar to those in other mammals. We found that BCs received numerous endbulb-like VGLUT1- and VGLUT2-immunopositive endings. In addition, they expressed glutamate AMPA (GluR2/3 and GluR4), NMDA (NR1), delta1/2 receptor subunits, and the α1 subunit of the glycine receptor. These receptor types and subunits mediate fast excitatory synaptic transmission from the cochlea and inhibitory neurotransmission from noncochlear inputs. Parvalbumin immunostaining and semithin sections showed that BC dendrites are oriented toward neighboring BC somas to form neuronal clusters. Within the cluster, the incoming inputs established multiple, divergent synaptic contacts. Thus, BCs were connected by specialized dendrosomatic and somasomatic membrane junctions. Our results indicate that the cytoarchitectural organization of BCs is well conserved between primates and other mammalian species.

Research highlights▶The ultrastructure of bushy cells of the primate is similar to other animal species. ▶The expression of key glutamate and glycine receptor subunits is as in rodents. ▶Bushy cells receive synaptic endings on the soma and dendrites. ▶Bushy cells make gap junctions with other bushy cells.Figure optionsDownload full-size imageDownload high-quality image (142 K)Download as PowerPoint slide