Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4342173 | Neuroscience | 2008 | 10 Pages |
Abstract
In situ zymography signal broadly co-localized with NeuN-positive cells, thus suggesting that proteolysis mainly occurs in neurons. Gelatinolytic activity was mainly detected in cell nuclei, marginally appearing in the cytosol only at later stages following the insult; we did not detect variations in gelatinolysis in the extracellular matrix. Finally, we report that pharmacological inhibition of MMPs by N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpenthanoyl]-l-tryptophan methylamide (GM6001) significantly reduces brain infarct volume induced by transient MCAo. Taken together our data underscore the crucial role of gelatinases during the early stages of reperfusion and further extend previous observations documenting the detrimental role of these enzymes in the pathophysiology of brain ischemia.
Keywords
GM6001MMPTTCDAPIECMMCAO2,3,5-triphenyltetrazolium chloride4′,6-diamidino-2-phenylindole dihydrochlorideu-PAReperfusion injuryNeuroinflammationmiddle cerebral artery occlusiontissue inhibitor of matrix metalloproteinaseTIMPIn situ zymographyBlood–brain barrierBBBStrokeurokinase plasminogen activatorExtracellular matrixmatrix metalloproteinaseMatrix metalloproteinases
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Authors
D. Amantea, M.T. Corasaniti, N.B. Mercuri, G. Bernardi, G. Bagetta,