Mef2 promotes spine elimination in absence of δ-catenin

δ-Catenin is a component of the cadherin–catenin cell adhesion complex and its loss has been implicated in the mental retardation associated with the Cri du chat syndrome. We have previously demonstrated that loss of δ-catenin in a murine model during development results in excessive spine and synaptic density and function. In order to examine the role of potential molecules that might cooperate with δ-catenin to regulate spine density, we focused on Mef2. Our data demonstrate that while loss of δ-catenin does not alter the expression levels of endogenous Mef2, expression of Mef2 in neurons that are knocked down for δ-catenin promotes spine elimination. These results establish a molecular mechanism by which excessive spines in the absence of δ-catenin may be eliminated and may point toward pharmacological therapy for the Cri du chat syndrome.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (116 K)Download as PowerPoint slideHighlights► Knockdown of δ-catenin promotes increase in spine density in hippocampal neurons. ► We examined if Mef2 cooperates with δ-catenin to regulate spine density. ► Mef2 rescues the excessive spine phenotype in δ-catenin knockdown neurons.