Protective role of N-trans-feruloyltyramine against β-amyloid peptide-induced neurotoxicity in rat cultured cortical neurons

Enhanced oxidative stress and inflammation play important roles in the pathogenesis of Alzheimer's disease (AD). Amyloid β-peptide (Aβ), a major component of amyloid plaques, is considered to have a causal role in the development and progress of AD by being the initiator of a pathological cascade leading to oxidative stress. The present study investigated the effect of N-trans-feruloyltyramine (NTF) purified from Polyalthia suberosa, an alkaloid shown to protect against oxidative stress and cell death. Pre-treatment of rat primary cortical cell cultures with 25–250 μM NTF significantly attenuated 10 μM Aβ1–42-induced neuronal death in a dose-dependent manner. Apoptotic cell death was demonstrated morphologically as well as by detection of the presence of activated caspase-3 and Bax, levels of which could be reduced by NTF pre-treatment. NTF also reduced production of reactive oxygen species induced by Aβ1–42. These findings suggest that the protective effect of NTF against Aβ1–42-induced neuronal death might be due to its antioxidative property.

► Pre-treatment of cortical cell cultures with NTF attenuated Aβ1–42-induced neuronal death. ► Cell death was demonstrated morphologically as well as by the presence of caspase-3 and Bax. ► NTF also reduced production of reactive oxygen species induced by Aβ1–42. ► Effect of NTF against Aβ1–42-induced neuronal death might be due to its antioxidative property. ► NTF has a potential role in alleviating neuronal apoptotic death associated with AD.