Glutamate-induced free radical formation in rat brain synaptosomes is not dependent on intrasynaptosomal mitochondria membrane potential

Article ID	Journal	Published Year	Pages	File Type
4344616	Neuroscience Letters	2012	5 Pages	PDF

Abstract

âº Glutamate induces oxidative stress in synaptosomes. âº Mitochondria are not involved in glutamate-induced ROS formation. âº NADPH oxidase and NO synthase are major sources for free radicals. âº Glutamate decreases membrane potential of intrasynaptosomal mitochondria. âº NMDA receptors are involved in glutamate-induced oxidative stress.

Keywords

DPI α-amino-3-hydroxy-5-methylisoxazole-4-propionate DCFDA MK-801 NO synthase N-methyl-d-aspartate NMDA CCCP NBQX AMPA NOS TRIS Dcf HEPES 2′,7′-dichlorodihydrofluorescein 2′,7′-dichlorodihydrofluorescein diacetate l-NAME N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid]Nω-nitro-l-arginine methyl ester hydrochloride ROS NADPH oxidase Synaptosomes Mitochondria carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone glutamate Reactive oxygen species