Effect of monolayer cells on sphere cells-Two types of cells that emerge during the neural differentiation of mouse embryonic stem cells

► Monolayer and sphere cells emerge during the neural differentiation of ES cells. ► Monolayer cells promote the survival and neuronal production of sphere cells. ► Monolayer cells reduce the apoptosis of sphere cells. ► Monolayer cells are not capable of differentiation into neurons or glia. ► Monolayer cells neither form tumor nor affect the tumorigenicity of sphere cells.