| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4345739 | Neuroscience Letters | 2010 | 5 Pages |
Recent studies have demonstrated the involvement of the sympathetic nerve system in bone metabolism. We have previously demonstrated the expression of adrenergic receptors in the human osteoblast SaM-1 cell line. The aim of this study was to reveal the function of these receptors in osteoblasts using electrophysiological methods. During whole-cell patch clamp recording, the application of noradrenaline reduced the currents induced by a voltage ramp, and the effect was larger in the positive potential range. Pretreatment with the selective α1B-adrenergic receptor antagonist chloroethylclonidine eliminated the inhibitory effect of noradrenaline. Using Cs-based pipette solution to block potassium channels, the inhibitory effect of noradrenaline disappeared. In contrast, the effect was unaffected in the presence of tetraethylammonium (TEA), a potassium channel blocker. These results suggest that noradrenaline suppresses Cs-sensitive and TEA-insensitive potassium channels via the α1B-adrenergic receptor in human osteoblasts.
Research highlights▶ The membrane potential is regulated by noradrenaline in the human osteoblast. ▶ Cs-sensitive and TEA-insensitive currents present in the human osteoblast. ▶ The stimulation of α1B-adrenoceptor suppresses potassium channels.
