GABA-immunoreactive photoreceptors in the retina of an anuran, Pelobates fuscus

We have recently started to unravel the retinal neurochemistry of an anuran species, the spadefoot toad (Pelobates fuscus), because of its unique lifestyle. The immunolabelling experiments included tests to localize the major inhibitory transmitter, γ-aminobutyric acid (GABA) to subsets of retinal neurons, using commercially available antibodies. Apart from the regular GABA-immunoreactive pattern observed formerly in other anurans, certain structures in the photoreceptor layer were also regularly labeled for GABA. The soma diameter of the labeled cells is 5–6 μm and the outer segment seems to be unlabeled. In resin-embedded preparations GABA-positive photoreceptor cells were identified as cones based on their sparse distribution and short outer segments. If these cells release GABA as a transmitter, it may act on the second order cells, from which certain horizontal and bipolar cells have functional GABA receptors. Alternatively, GABA may influence the cones themselves through autoreceptors.