Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4349801 | Neuroscience Letters | 2007 | 6 Pages |
Abstract
Long-term potentiation (LTP) is a persistent increase in the strength of synaptic transmission triggered by neuronal activity. Here, we submitted hippocampal slices to a perfusion of forskolin and IBMX, which induces a long-lasting LTP (>4Â h) (L-LTP). We separated the proteins of the CA1 region by two-dimensional gel electrophoresis (2-DE). We then immunoblotted them using an anti-p-Tyr antibody. We found a protein whose tyrosine phosphorylation was unchanged 10Â min after LTP induction but was dramatically increased after 1Â h, dropping back to its baseline after 4Â h. This protein was identified as rabphilin using matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). We also demonstrated that genistein, an inhibitor of tyrosine phosphorylation, prevented the development of the late phase of electrically-induced L-LTP. Our results suggest that rabphilin, a protein present in presynaptic terminals, could play a role in the late phase of L-LTP.
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Authors
Brigitte Capron, Ruddy Wattiez, Christian Sindic, Emile Godaux, Laurence Ris,