Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4350579 | Neuroscience Letters | 2006 | 5 Pages |
Abstract
Physostigmine (Phy), a reversible inhibitor of acetylcholine (ACh) esterase (AChE), may also act as a low potency agonist and a modulator of the nicotinic receptor. The actions of Phy on mouse muscle nicotinic receptors in the COS-7 cell line were studied by the patch-clamp technique. Currents were recorded in the whole-cell mode 3-7 days after cell transfection by plasmids coding αβγδ combination of receptor subunits. The application of ACh to cells clamped at â10 mV produced inward currents which displayed desensitization. The application of Phy in concentrations up to 1 Ã 10â3 M did not give reliable specific whole-cell membrane responses. The application of Phy in concentrations of 10â6-10â4 M together with ACh modulated the amplitude; accelerated desensitization of currents induced by ACh and increased the final extent of desensitization in a concentration-dependent manner. This finding is in contrast to the suppression and slowing down of desensitization by Phy and 1-methyl-galanthamine observed in Torpedo receptors.
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Authors
Lucie Svobodová, Jan Krůšek, TomáÅ¡ Hendrych, FrantiÅ¡ek VyskoÄil,