Administration of antisense DNA for GPR39-1b causes anxiolytic-like responses and appetite loss in rats

The G protein-coupled receptor 39-b (GPR39-1b) is a splice variant of which is expressed in the central nervous and gastrointestinal systems. Previously, GPR39-1b was proposed to be the receptor for obestatin, but current evidence does not support this hypothesis. The purpose of the present work was to identify the role of GPR39-1b in anxiety and eating behaviors. Antisense oligonucleotides were infused at a constant rate into the cerebral lateral ventricles of rats and their effect on anxiety-like behavior and food intake was monitored. GPR39-1b antisense oligonucleotides produced anxiolytic-like effects in the elevated-plus maze test and in the black and white box test. Antisense oligonucleotides also decreased food intake. These results indicate that inhibition of GPR39-1b induces a decrease in anxiety-related behaviors and disturbs appetite.

► We examined the effect of antisense oligonucleotides for GPR39-1b, which is the receptor for obestatin, on anxiety and food intake. ► We infused GPR39-1b antisense oligonucleotides into the cerebral lateral ventricles of rats. ► The antisense oligonucleotides produced anxiolytic-like effects in the elevated-plus maze test and the black and white test. ► The antisense oligonucleotides decreased food intake.