Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4352248 | Neuroscience Research | 2010 | 4 Pages |
Abstract
Bioluminescence imaging is a powerful tool for examining gene expression in living animals. Previously, we reported that exogenous DNA could be successfully delivered into neurons in the newly hatched chick brain using electroporation. Here, we show the in vivo bioluminescence imaging of c-fos promoter activity and its upregulation, which is associated with filial imprinting. The upregulation of c-fos gene expression correlated with both the strength of the chicks’ approach activity to the training object and the acquisition of memory. The present technique should be a powerful tool for analyzing the time changes in neural activity of certain brain areas in real-time during memory formation, using brains of living animals.
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Neuroscience
Neuroscience (General)
Authors
Shinji Yamaguchi, Eiji Iikubo, Naoki Hirose, Takaaki Kitajima, Sachiko Katagiri, Ai Kawamori, Ikuko Fujii-Taira, Toshiya Matsushima, Koichi J. Homma,