Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4352477 | Neuroscience Research | 2007 | 4 Pages |
Abstract
Neuropeptide FF (NPFF) agonists counteract the cellular opioid actions. We demonstrate for the first time a biochemical anti-opioid effect of NPFF receptors in the rat spinal cord by using the [35S]GTPγS binding assay in autoradiography. The mu agonist DAMGO as well as the potent and selective NPFF2 agonist dNPA, stimulated [35S]GTPγS binding at different optimal GDP concentrations. dNPA decreased the effects induced by DAMGO alone; the maximal of G-protein coupling was decreased but not the potency of opioid agonist. We conclude that NPFF2 receptors are coupled to G-protein in the rat spinal cord and could exert a molecular anti-opioid effect.
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Christine Gouardères, Jean-Marie Zajac,