Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4352578 | Neuroscience Research | 2007 | 8 Pages |
Abstract
Retinoic acid-inducible gene-I (RIG-I) mediates part of the cell signaling in response to viral infection. Polyinosinic-polycytidilic acid (poly IC) is a synthetic double-stranded RNA (dsRNA) and mimics viral infection when applied to cell cultures. The CC chemokine, RANTES (regulated on activation, normal T-cell expressed and secreted), is a potent attractant for inflammatory cells such as memory T-lymphocytes, monocytes and eosinophils. In the present study, we demonstrated that poly IC enhances the expression of RIG-I in U373MG human astrocytoma cells. The RNA interference of RIG-I resulted in the suppression of the poly IC-induced RANTES expression. Pretreatment of the cells with SB203580, an inhibitor of p38 mitogen-activated protein kinase, and dexamethasone inhibited the poly IC-induced expression of RIG-I. Furthermore, poly IC upregulated RIG-I in normal human astrocytes in culture and the in vivo injection of poly IC into the striatum of the mouse brain induced the expression of RIG-I in astrocytes. We conclude that RIG-I may be involved in immune reactions against viral infection, at least in part, through the regulation of RANTES expression in astrocytes.
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Hidemi Yoshida, Tadaatsu Imaizumi, Sung Joong Lee, Kunikazu Tanji, Hirotaka Sakaki, Tomoh Matsumiya, Akira Ishikawa, Kageaki Taima, Ei Yuzawa, Fumiaki Mori, Koichi Wakabayashi, Hiroto Kimura, Kei Satoh,