Effects of GABAB-agonist on rat hypothalamic neurons: Functional antagonism with μ-receptor agonist

Extracellular and whole-cell patch clamp recordings were made from neurons in slices of the preoptic area/anterior hypothalamus (PO/AH) of rats, to investigate the effects of the GABAB-receptor agonist baclofen on neuronal response characteristics, as well as its interactions with μ-opioid receptor agonist PL-017 on the level of central temperature controller. Baclofen decreased tonic activity (firing rate) in all types of neurons, but increased temperature sensitivity (temperature coefficient, TC) in warm-sensitive neurons. The decrease in firing rate during baclofen application was accompanied with significant membrane hyperpolarization and decrease of input resistances. The tonic activity (in all type of PO/AH neurons), as well as the temperature sensitivity (in warm-sensitive neurons), were inhibited by μ-opioid receptor agonist PL-017. Remarkably, the effect on temperature sensitivity was abolished and absence of synergism in regard to firing rate decrease occurred, when baclofen and PL-017 were applied simultaneously. Our results are step of understanding the complicated mechanisms of action of neurotransmitters and their interactions on the level of central temperature controller—the neurons of the PO/AH.