Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4353200 | Neuroscience Research | 2006 | 9 Pages |
Abstract
To clarify the role of phosphorylated extracellular signal-regulated kinases (pERK1/2) in the neuroprotection of limb ischemic preconditioning (LIP) in rats, we investigated the expression of pERK1/2 using Western blot and flow cytometry in the hippocampus after LIP and the effect of pERK1/2 inhibitor PD 98059 on the neuroprotection of LIP against delayed neuronal death (DND) in the CA1 hippocampus normally induced by severe ischemic insult. It demonstrated that pERK1/2 in the hippocampus increased after LIP. In the CA1 hippocampus, ERK1/2 activation began to increase at 6Â h and reached peak at 12Â h after LIP, and decreased to sham level at 5Â d after LIP. On the other hand, in the CA3/DG, pERK1/2 enhanced at 1Â d, reached peak at 3Â d, and lasted to 5Â d after LIP. Pretreatement with PD 98059 before LIP blocked the neuroprotection of LIP in a dose-dependent manner. These findings supported that the upregulation of pERK1/2 in the CA1 hippocampus contributed to the neuroprotection of LIP against DND normally caused by the brain ischemic insult.
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Authors
Rui-Li Jin, Wen-Bin Li, Qing-Jun Li, Min Zhang, Xiao-hui Xian, Xiao-Cai Sun, Hong-Gang Zhao, Jie Qi,