| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4355525 | Hearing Research | 2010 | 6 Pages |
Abstract
⺠We have developed a liquid chromatography-mass spectrometry method for tissue sections embedded with celloidin. ⺠Five different tissue extraction methods were used to evaluate formalin de-cross-linking. ⺠Four methods were used to evaluate celloidin removal for liquid chromatography-mass spectrometry. ⺠The methods judged best were applied to formalin-fixed celloidin-embedded sections of mouse inner ear samples, and human inner ear and human otic capsule bone sections. ⺠Proteins from all major cellular components were detected in the formalin-fixed celloidin-embedded archival human temporal bone sections.
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Authors
Antti A. Aarnisalo, Karin M. Green, Jennifer O'Malley, Chadi Makary, Joe Adams, Saumil N. Merchant, James E. Evans,