Review: Cytological characteristics of commissural and tuberculo-ventral neurons in the rat dorsal cochlear nucleus

The goal of the present review is to summarize the main ultrastructural and immunocytochemical characteristics for glycine and GABA in commissural (COM) and tuberculo-ventral neurons (TV) of the DCN. These neurons are localized in similar areas of the DCN multipolar but are connected to different targets. About 2/3rd of COM-neurons are large to bipolar neurons, mainly glycinergic, often GABA-ergic, with scarce ergastoplasm and axo-somatic boutons. About 1/3rd of COM-neurons are glycine and GABA-negative, and show little ergastoplasm and synaptic coverage. Occasional giant COM-neurons are glycine-positive and GABA-negative, and are covered with synaptic boutons. Other infrequent large neurons, rich in dense core vesicles, glycine- and GABA-negative, are most covered with boutons. TV-neurons are most glycinergic but 9% are glycine-negative. They have little ergastoplasm and a developed Golgi apparatus. Axo-somatic terminals are scarce and mainly contain flat and pleomorphic vesicles, glycine and sometimes GABA (inhibitory). TV-neurons receive a lower number of boutons than COM, which contain mainly flat-pleomorphic terminals. Putative COM-inhibitory boutons contact excitatory pyramidal and giant neurons (monosynaptic inhibition). Some putative inhibitory COM-terminals contact inhibitory cartwheel and tuberculo-ventral neurons. This indicates direct disinhibition and therefore excitation in the DCN (di-three-synaptic). Putative COM-mossy fibers reach the granule areas of the DCN, including unipolar brush cell dendrites, another possible excitatory commissural pathway.