| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4358569 | Research in Microbiology | 2014 | 10 Pages |
A novel marine bacterium, strain LBS2T was isolated from eggs carried on pleopods of the spiny lobster collected from Andaman Sea. Heterotrophic growth occurred at 1–7% NaCl. 16S rRNA gene sequence similarity revealed the strain LBS2T belonged to the genus Vibrio and showed above 97% similarity with eight type strains of the genus Vibrio. Multilocus analysis based on ftsZ, gapA, gyrB, mreB, pyrH recA, rpoA, and topA revealed LBS2T formed a separate cluster with Vibrio ponticus DSM 16217T with 89.8% multilocus gene sequence similarity. However, strain LBS2T is distantly related with other members of the Scophthalmi clade in terms of 16S rRNA signatures, phenotypic variations and multilocus gene sequence similarity, for which we propose LBS2T belongs to a new clade i.e. Ponticus clade with V. ponticus DSM 16217T as the representative type strain of the clade. DNA–DNA homologies between strain LBS2T and closely related strains were well below 70%. DNA G + C content was 45.3 mol%. On the basis of our polyphasic study, strain LBS2T represents a novel species of the genus Vibrio, for which the name Vibrio panuliri sp. nov. is proposed. The type strain is LBS2T (= JCM 19500T = DSM 27724T = LMG 27902T).
