Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4358923 | Research in Microbiology | 2010 | 8 Pages |
Abstract
The worldwide emergence of antibiotic-resistant bacteria poses a serious threat to human health. In addition to the difficulties in controlling infectious diseases, the phenotype of resistance can generate metabolic changes which, in turn, can interfere with host-pathogen interactions. The aim of the present study was to identify changes in the subproteome of a laboratory-derived piperacillin/tazobactam-resistant strain of Escherichia coli (minimal inhibitory concentration [MIC]Â =Â 128Â mg/L) as compared with its susceptible wild-type strain E. coli ATCC 25922 (MICÂ =Â 2Â mg/L) using 2-D fluorescence difference gel electrophoresis (2D-DIGE) followed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF MS). In the resistant strain, a total of 12 protein species were increased in abundance relative to the wild-type strain, including those related to bacterial virulence, antibiotic resistance and DNA protection during stress. Fourteen proteins were increased in abundance in the wild-type strain compared to the resistant strain, including those involved in glycolysis, protein biosynthesis, pentose-phosphate shunt, amino acid transport, cell division and oxidative stress response. In conclusion, our data show overall changes in the subproteome of the piperacillin/tazobactam-resistant strain, reporting for the first time the potential role of a multidrug efflux pump system in E. coli resistance to piperacillin/tazobactam.
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Authors
Kênia Valéria dos Santos, Cláudio Galuppo Diniz, Luciano de Castro Veloso, Hélida Monteiro de Andrade, Mario da Silva Giusta, Simone da Fonseca Pires, Agenor Valadares Santos, Ana Carolina Morais Apolônio, Maria Auxiliadora Roque de Carvalho,