| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4359027 | Research in Microbiology | 2009 | 7 Pages |
Abstract
The work described here continues our studies of the effects of subinhibitory concentrations of antibiotics on SOS and DNA repair gene expression in Staphylococcus aureus. Mitomycin C and the new-generation fluoroquinolone moxifloxacin induced expression of SOS response genes (lexA, recA, sosA, and umuC) in a ciprofloxacin-resistant CiprI strain of S. aureus. To examine phenotypic changes in Cipr strains mutated in CIP targets (GrlA and/or GyrA), we used Biolog Phenotype MicroArraysâ¢. Two other Cipr strains mutated in the norA promoter region were used to study the effects of subinhibitory concentrations of DNA-damaging antibiotics on norA expression. We show that mitomycin C and moxifloxacin induced overexpression of the norA gene in Cipr strains. Finally, we confirm that subinhibitory concentrations of CIP increase mutation rates in S. aureus.
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Authors
Lili R. Mesak, Julian Davies,