| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4364237 | International Biodeterioration & Biodegradation | 2016 | 13 Pages |
•The waste management of mackerel wastewater was demonstrated for full reutilization.•Major degradation mechanism in this study was the depolymerization of protein.•Effects of the amino acids on antioxidant activity were confirmed using 2D-TLC.•Culture supernatants showed antimicrobial and DNA protective activities.•The remaining culture broth containing microbes could be used as a biofertilizer.
To reutilize mackerel wastewater (MWW) generated from mackerel processing plants, aerobic biodegradation of MWW was conducted in a 3-l reactor by using mixed microbes. During the first 24 h, biodegradation occurred actively, and the dissolved oxygen level, pH, and oxidation–reduction potential decreased with an increase in cell number. Then, the fishy smell started to disappear. The 42-h culture supernatant displayed high antioxidant activities, as indicated by the following results: 88.7% 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity, 99.7% 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, 96% hydroxyl radical scavenging activity, and 0.35 reducing power at A700. The high antioxidant activities were attributed to specific amino acids, and this was confirmed using two-dimensional thin-layer chromatography. Moreover, the culture supernatants displayed antimicrobial and DNA protective activities and weak antifungal activity, enhancing the reutilization value of MWW. The remaining culture broth after MWW biodegradation was found to be phytotoxin-free, and it could be used as a biofertilizer on the basis of hydroponic culture of red bean and barley. Overall, advanced waste management of MWW was demonstrated.
