Biodegradation of Bisphenol A by the tolerant bacterial species isolated from coastal regions of Chennai, Tamil Nadu, India

•Isolation of bacterial species with high Bisphenol A tolerance.•Bacterial strains isolated from Seawater and sediment sample collected from coastal regions of Chennai, Tamilnadu, India.•Potential Bisphenol A degrading efficiency of bacterial strains at 1000 ppm.•Sodium glutamate as co-substrate accelerate the degradation efficiency.•Molecular identification of three bacterial strains with 99% homology using 16S rRNA analysis.

Bacterial strains having high BPA tolerance were isolated from seawater and sediment samples collected from coastal regions of Chennai, Tamil Nadu, India. Three different bacteria, designated as K-6, K-8, and KU-3, showed the best degradation efficiency of 78 ± 4%, 81 ± 3%, and 74 ± 2%, respectively, at 150 rpm (12 days at 37 °C) in mineral medium supplemented with 1000 ppm BPA. The best degradation was obtained at pH 7.0 and it subsequently decreased with alteration of pH. Sodium glutamate as co-substrate accelerated the degradation efficiency 85 ± 3% (K-8), 83 ± 2% (K6), and 81 ± 3% (KU-3). The bacterial strains K-6, K-8, and KU-3 exhibited degradation rates of 78%, 70%, and 61%, respectively, for 150 rpm (15 days at 37 °C) in the seawater containing BPA (1000 ppm) as a sole carbon and energy source. Residual BPA was analyzed by gas chromatography. These strains were identified as Pseudomonas sp. strain KU1, Pseudomonas sp. strain KU2, and Bacillus sp. strain KU3 by partial 16S rDNA analysis.

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