Article ID Journal Published Year Pages File Type
4364999 International Biodeterioration & Biodegradation 2014 6 Pages PDF
Abstract

•Biodegradation of high-molecular weight PAH pyrene using fusant strain.•F14 has higher degradation ability on pyrene than its parental strain.•The substrate interactions in binary and ternary mixtures of pyrene were studied.•Investigating the kinetics of pyrene degradation in binary and ternary mixtures.•The metabolite of 4, 5-dihydropyrene was detected during pyrene degradation.

The aim of the present study was to investigate pyrene degradation by a functional strain F14, which was constructed through protoplast fusion between Sphingomonas sp. GY2B and Pseudomonas sp. GP3A. The ability of F14 to degrade pyrene increased from 18% to 46% with the decrease of pyrene concentration from 100 mg L−1 to 15 mg L−1 within 10 days. When pyrene was in binary mixture with phenanthrene or naphthalene, about 42% and 78% of pyrene were biodegraded by F14, respectively, indicating the presence of naphthalene or phenanthrene enhanced pyrene degradation, and the enhanced effect of 2-ring naphthalene was more obvious than that of 3-ring phenanthrene. Detection of metabolites 4, 5-dihydropyrene during pyrene degradation further confirmed the degradation capability of fusion strain F14. The present study showed that fusion strain F14 have a promising application in bioremediation of PAHs contaminated environment.

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