Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4371107 | Experimental Parasitology | 2014 | 8 Pages |
Abstract
•T. gondii tachyzoites exposed to UV for 1 min still have intact membranes.•With 1 min UV exposure, tachyzoites are unable to replicate in vitro or produce parasite cysts in vivo.•UV-inactivated tachyzoites can stimulate an IgG response but not cytokine response in vivo.
We developed a protocol to inactivate Toxoplasma gondii (T. gondii) tachyzoites employing 1 min of ultraviolet (UV) exposure. We show that this treatment completely inhibited parasite replication and cyst formation in vitro and in vivo but did not affect the induction of a robust IgG response in mice. We propose that our protocol can be used to study the contribution of the humoral immune response to rodent behavioral alterations following T. gondii infection.
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Authors
Geetha Kannan, Emese Prandovszky, Curtis B. Steinfeldt, Kristin L. Gressitt, ChunXia Yang, Robert H. Yolken, Emily G. Severance, Lorraine Jones-Brando, Mikhail V. Pletnikov,